Last updated: 2024-10-19

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Knit directory: DEanalysis/

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File Version Author Date Message
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html af88524 C-HW 2024-10-15 Build site.
Rmd 07b55b4 C-HW 2024-10-15 update Seurat result
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html bc13544 C-HW 2023-12-04 Build site.
Rmd 39196a3 C-HW 2023-12-04 fix log2FC sign
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Rmd 3803697 C-HW 2023-12-01 upload rmd
html eeb5d79 C-HW 2023-11-30 update B cells and data2
html 85fc2fe C-HW 2023-09-12 update
html d7d838c C-HW 2023-08-11 update graph
html 382e3cd C-HW 2023-08-03 update data2 heatmap
html fc4ab47 C-HW 2023-08-03 data2 results

Data summary

Version Author Date
4602dd7 C-HW 2024-10-14
3d93462 C-HW 2023-12-01
632be33 C-HW 2023-12-01
eeb5d79 C-HW 2023-11-30
85fc2fe C-HW 2023-09-12
d7d838c C-HW 2023-08-11
fc4ab47 C-HW 2023-08-03

Number of DEGs from each method

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14
42900f0 C-HW 2023-12-01
eeb5d79 C-HW 2023-11-30
d7d838c C-HW 2023-08-11
fc4ab47 C-HW 2023-08-03

Upset plot

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14
bc13544 C-HW 2023-12-04
42900f0 C-HW 2023-12-01
eeb5d79 C-HW 2023-11-30
d7d838c C-HW 2023-08-11
fc4ab47 C-HW 2023-08-03

Volcano plot

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14

Histogram of p-value/adj.p-value

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
632be33 C-HW 2023-12-01
fc4ab47 C-HW 2023-08-03

P-Value comparison across different methods

Version Author Date
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15

Log2 fold change comparison across different methods

Version Author Date
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
42900f0 C-HW 2023-12-01
3d93462 C-HW 2023-12-01
632be33 C-HW 2023-12-01
eeb5d79 C-HW 2023-11-30
85fc2fe C-HW 2023-09-12
d7d838c C-HW 2023-08-11

Violin plot of log2mean of DEGs

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
42900f0 C-HW 2023-12-01
eeb5d79 C-HW 2023-11-30
d7d838c C-HW 2023-08-11

Violin plot of gene expression frequency of DEGs

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
eeb5d79 C-HW 2023-11-30

Heatmap of top DEGs

Poisson-glmm DEGs

Version Author Date
9bd93f2 C-HW 2024-10-19
4602dd7 C-HW 2024-10-14

Additional DEGs from other methods

Wilcox

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14

pb-DESeq2

Version Author Date
9bd93f2 C-HW 2024-10-19
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
bc13544 C-HW 2023-12-04
42900f0 C-HW 2023-12-01
3d93462 C-HW 2023-12-01
632be33 C-HW 2023-12-01
85fc2fe C-HW 2023-09-12
d7d838c C-HW 2023-08-11
382e3cd C-HW 2023-08-03
fc4ab47 C-HW 2023-08-03

MMpoisson

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
3d93462 C-HW 2023-12-01
632be33 C-HW 2023-12-01
85fc2fe C-HW 2023-09-12
d7d838c C-HW 2023-08-11
fc4ab47 C-HW 2023-08-03

MA plot

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
3d93462 C-HW 2023-12-01
eeb5d79 C-HW 2023-11-30
d7d838c C-HW 2023-08-11

Enrichment analysis

Poisson-glmm DEGs

All DEGs

Version Author Date
9bd93f2 C-HW 2024-10-19
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
9e46095 C-HW 2023-12-04
917ddb4 C-HW 2023-12-03
3d93462 C-HW 2023-12-01

Version Author Date
9bd93f2 C-HW 2024-10-19
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
bc13544 C-HW 2023-12-04
9e46095 C-HW 2023-12-04
917ddb4 C-HW 2023-12-03
35e3430 C-HW 2023-12-03
3d93462 C-HW 2023-12-01
eeb5d79 C-HW 2023-11-30

Up-regulated DEGs

Version Author Date
9bd93f2 C-HW 2024-10-19
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
9e46095 C-HW 2023-12-04
917ddb4 C-HW 2023-12-03
3d93462 C-HW 2023-12-01

Version Author Date
9bd93f2 C-HW 2024-10-19
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
bc13544 C-HW 2023-12-04
9e46095 C-HW 2023-12-04
917ddb4 C-HW 2023-12-03
35e3430 C-HW 2023-12-03
42900f0 C-HW 2023-12-01
e3d28f8 C-HW 2023-12-01
3d93462 C-HW 2023-12-01
eeb5d79 C-HW 2023-11-30

Down-regulated DEGs

Version Author Date
9bd93f2 C-HW 2024-10-19
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
3d93462 C-HW 2023-12-01

Version Author Date
9bd93f2 C-HW 2024-10-19
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
2a17159 C-HW 2023-12-05
bc13544 C-HW 2023-12-04
9e46095 C-HW 2023-12-04
917ddb4 C-HW 2023-12-03
35e3430 C-HW 2023-12-03
42900f0 C-HW 2023-12-01
e3d28f8 C-HW 2023-12-01
3d93462 C-HW 2023-12-01
84814ca C-HW 2023-12-01

pb-DESeq2 DEGs

All DEGs

Version Author Date
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
2a17159 C-HW 2023-12-05
bc13544 C-HW 2023-12-04
9e46095 C-HW 2023-12-04
917ddb4 C-HW 2023-12-03
35e3430 C-HW 2023-12-03
42900f0 C-HW 2023-12-01
e3d28f8 C-HW 2023-12-01
3d93462 C-HW 2023-12-01
84814ca C-HW 2023-12-01

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
3d93462 C-HW 2023-12-01
84814ca C-HW 2023-12-01

Up-regulated DEGs

Version Author Date
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
bc13544 C-HW 2023-12-04
9e46095 C-HW 2023-12-04
35e3430 C-HW 2023-12-03

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15

Down-regulated DEGs

Version Author Date
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
2a17159 C-HW 2023-12-05
bc13544 C-HW 2023-12-04
9e46095 C-HW 2023-12-04
917ddb4 C-HW 2023-12-03
35e3430 C-HW 2023-12-03

Version Author Date
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15

MMpoisson DEGs

All DEGs

Version Author Date
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
2a17159 C-HW 2023-12-05
bc13544 C-HW 2023-12-04
9e46095 C-HW 2023-12-04
917ddb4 C-HW 2023-12-03
35e3430 C-HW 2023-12-03

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15

Up-regulated DEGs

Version Author Date
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15
bc13544 C-HW 2023-12-04
9e46095 C-HW 2023-12-04
35e3430 C-HW 2023-12-03

Version Author Date
9bd93f2 C-HW 2024-10-19
af88524 C-HW 2024-10-15
4602dd7 C-HW 2024-10-14
a0d4555 C-HW 2023-12-15

Down-regulated DEGs


R version 4.4.1 (2024-06-14)
Platform: x86_64-apple-darwin20
Running under: macOS Monterey 12.6

Matrix products: default
BLAS:   /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRblas.0.dylib 
LAPACK: /Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/lib/libRlapack.dylib;  LAPACK version 3.12.0

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

time zone: America/Chicago
tzcode source: internal

attached base packages:
[1] stats4    stats     graphics  grDevices utils     datasets  methods  
[8] base     

other attached packages:
 [1] UpSetR_1.4.0                SeuratObject_5.0.2         
 [3] sp_2.1-4                    pathview_1.44.0            
 [5] org.Hs.eg.db_3.19.1         AnnotationDbi_1.66.0       
 [7] enrichplot_1.24.4           clusterProfiler_4.12.6     
 [9] reshape_0.8.9               gridExtra_2.3              
[11] pheatmap_1.0.12             SingleCellExperiment_1.26.0
[13] SummarizedExperiment_1.34.0 Biobase_2.64.0             
[15] GenomicRanges_1.56.2        GenomeInfoDb_1.40.1        
[17] IRanges_2.38.1              S4Vectors_0.42.1           
[19] BiocGenerics_0.50.0         MatrixGenerics_1.16.0      
[21] matrixStats_1.4.1           ggpubr_0.6.0               
[23] dplyr_1.1.4                 ggplot2_3.5.1              

loaded via a namespace (and not attached):
  [1] splines_4.4.1           later_1.3.2             bitops_1.0-9           
  [4] ggplotify_0.1.2         tibble_3.2.1            R.oo_1.26.0            
  [7] polyclip_1.10-7         graph_1.82.0            XML_3.99-0.17          
 [10] lifecycle_1.0.4         httr2_1.0.5             rstatix_0.7.2          
 [13] rprojroot_2.0.4         globals_0.16.3          lattice_0.22-6         
 [16] MASS_7.3-61             backports_1.5.0         magrittr_2.0.3         
 [19] sass_0.4.9              rmarkdown_2.28          jquerylib_0.1.4        
 [22] yaml_2.3.10             httpuv_1.6.15           spam_2.11-0            
 [25] cowplot_1.1.3           DBI_1.2.3               RColorBrewer_1.1-3     
 [28] abind_1.4-8             zlibbioc_1.50.0         purrr_1.0.2            
 [31] R.utils_2.12.3          ggraph_2.2.1            RCurl_1.98-1.16        
 [34] yulab.utils_0.1.7       tweenr_2.0.3            rappdirs_0.3.3         
 [37] git2r_0.33.0            GenomeInfoDbData_1.2.12 ggrepel_0.9.6          
 [40] listenv_0.9.1           tidytree_0.4.6          parallelly_1.38.0      
 [43] codetools_0.2-20        DelayedArray_0.30.1     DOSE_3.30.5            
 [46] ggforce_0.4.2           tidyselect_1.2.1        aplot_0.2.3            
 [49] UCSC.utils_1.0.0        farver_2.1.2            viridis_0.6.5          
 [52] jsonlite_1.8.9          progressr_0.14.0        tidygraph_1.3.1        
 [55] Formula_1.2-5           ggnewscale_0.5.0        tools_4.4.1            
 [58] treeio_1.28.0           Rcpp_1.0.13             glue_1.8.0             
 [61] SparseArray_1.4.8       xfun_0.48               qvalue_2.36.0          
 [64] withr_3.0.1             fastmap_1.2.0           fansi_1.0.6            
 [67] digest_0.6.37           R6_2.5.1                gridGraphics_0.5-1     
 [70] colorspace_2.1-1        GO.db_3.19.1            RSQLite_2.3.7          
 [73] R.methodsS3_1.8.2       utf8_1.2.4              tidyr_1.3.1            
 [76] generics_0.1.3          data.table_1.16.2       graphlayouts_1.2.0     
 [79] httr_1.4.7              S4Arrays_1.4.1          scatterpie_0.2.4       
 [82] whisker_0.4.1           pkgconfig_2.0.3         gtable_0.3.5           
 [85] blob_1.2.4              workflowr_1.7.1         XVector_0.44.0         
 [88] shadowtext_0.1.4        htmltools_0.5.8.1       carData_3.0-5          
 [91] dotCall64_1.2           fgsea_1.30.0            scales_1.3.0           
 [94] png_0.1-8               ggfun_0.1.6             knitr_1.48             
 [97] rstudioapi_0.16.0       reshape2_1.4.4          nlme_3.1-166           
[100] cachem_1.1.0            stringr_1.5.1           parallel_4.4.1         
[103] pillar_1.9.0            grid_4.4.1              vctrs_0.6.5            
[106] promises_1.3.0          car_3.1-3               Rgraphviz_2.48.0       
[109] evaluate_1.0.1          KEGGgraph_1.64.0        cli_3.6.3              
[112] compiler_4.4.1          rlang_1.1.4             crayon_1.5.3           
[115] future.apply_1.11.2     ggsignif_0.6.4          labeling_0.4.3         
[118] plyr_1.8.9              fs_1.6.4                stringi_1.8.4          
[121] viridisLite_0.4.2       BiocParallel_1.38.0     munsell_0.5.1          
[124] Biostrings_2.72.1       lazyeval_0.2.2          GOSemSim_2.30.2        
[127] Matrix_1.7-0            patchwork_1.3.0         bit64_4.5.2            
[130] future_1.34.0           KEGGREST_1.44.1         highr_0.11             
[133] igraph_2.0.3            broom_1.0.7             memoise_2.0.1          
[136] bslib_0.8.0             ggtree_3.12.0           fastmatch_1.1-4        
[139] bit_4.5.0               ape_5.8                 gson_0.1.0